[ome-users] FLIMfit is not fitting B-H data nor PQ data

[Munro, Ian]

Hi Jan

I simply meant that the SNR of the fitted lifetime is much lower using the tail-fitting approach as you discard a lot of the light.

Would it be possible to see one of your data sets & associated irf ?

Perhaps we could then work out what the problem is.

Best

Ian


On 18 Sep 2014, at 13:36, Borst, Jan Willem <janwillem.borst at wur.nl<mailto:janwillem.borst at wur.nl>> wrote:

Hi Ian

We have IRF measured and have tried to use it and have tried it without.
No fitting possible
S/N is pretty good especially if you use bin option.

Talk to you later

JW
On 18 Sep 2014, at 14:09, Munro, Ian <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:

Dear Jan

I guess the first question is “ do you have a measured irf ? “.
If not , then you may , if your data is truly mono-exponential and there are enough photons,. be able to  get a good lifetime by using the tail- fitting
approach that I describe here:

http://lists.openmicroscopy.org.uk/pipermail/flimfit-users/2014-September/000005.html

For anything other than pure mono-exponential decays and  in order to maximise your SNR we strongly suggest measuring an irf
along with each experiment.

Some information about FLIMfit and irfs is available at https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough (about half-way
doen the page.)

All the best.

Ian

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