[ome-users] [FLIMfit-users] FLIMfit is not fitting B-H data nor PQ data

[Munro, Ian]

Hi Jan

No problem/. Whenever you have the time.

Best

Ian 


On 18 Sep 2014, at 13:49, Borst, Jan Willem <janwillem.borst at wur.nl> wrote:

> Hi Ian
> 
> Would be great, but I have to postpone it to next week as I am on travelling coming days and have to work on a proposal ……
> 
> Thanks. 
> 
> JW
> On 18 Sep 2014, at 14:44, Munro, Ian <i.munro at imperial.ac.uk> wrote:
> 
>> Hi Jan
>> 
>> I simply meant that the SNR of the fitted lifetime is much lower using the tail-fitting approach as you discard a lot of the light.
>> 
>> Would it be possible to see one of your data sets & associated irf ?
>> 
>> Perhaps we could then work out what the problem is.
>> 
>> Best 
>> 
>> Ian
>> 
>> 
>> On 18 Sep 2014, at 13:36, Borst, Jan Willem <janwillem.borst at wur.nl> wrote:
>> 
>>> Hi Ian
>>> 
>>> We have IRF measured and have tried to use it and have tried it without.
>>> No fitting possible
>>> S/N is pretty good especially if you use bin option.
>>> 
>>> Talk to you later
>>> 
>>> JW
>>> On 18 Sep 2014, at 14:09, Munro, Ian <i.munro at imperial.ac.uk> wrote:
>>> 
>>>> Dear Jan
>>>> 
>>>> I guess the first question is “ do you have a measured irf ? “.
>>>> If not , then you may , if your data is truly mono-exponential and there are enough photons,. be able to  get a good lifetime by using the tail- fitting  
>>>> approach that I describe here:
>>>> 
>>>> http://lists.openmicroscopy.org.uk/pipermail/flimfit-users/2014-September/000005.html
>>>> 
>>>> For anything other than pure mono-exponential decays and  in order to maximise your SNR we strongly suggest measuring an irf 
>>>> along with each experiment.
>>>> 
>>>> Some information about FLIMfit and irfs is available at https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough (about half-way 
>>>> doen the page.)
>>>> 
>>>> All the best.
>>>> 
>>>> Ian
>>>> 
>>>> 
>>>> 
>>>> 
>>>> 
>>>> On 18 Sep 2014, at 12:48, Borst, Jan Willem <janwillem.borst at wur.nl> wrote:
>>>> 
>>>>> Dear all
>>>>> 
>>>>> We are trying to use FLIMfit 4.7.0 for analysing FLIM data.
>>>>> We are able to load B-H data as well as PQ data (bin format) and obtain the intensity image.
>>>>> Selection of a pixel displays the decay curve but not good fit is obtained.
>>>>> 
>>>>> Can someone maybe post guidelines which settings should be used?
>>>>> There is FLIMfit documentation but that does not give a protocol of the steps to take.
>>>>> 
>>>>> All info is welcome.
>>>>> 
>>>>> Best
>>>>> 
>>>>> JW
>>>>> 
>>>>> 
>>>>> _______________________________________________
>>>>> FLIMfit-users mailing list
>>>>> FLIMfit-users at lists.openmicroscopy.org.uk
>>>>> http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users
>>>> 
>>> 
>> 
>