[ome-users] FLIMfit is not fitting B-H data nor PQ data

[Munro, Ian]

Dear Jan

I guess the first question is “ do you have a measured irf ? “.
If not , then you may , if your data is truly mono-exponential and there are enough photons,. be able to  get a good lifetime by using the tail- fitting
approach that I describe here:

http://lists.openmicroscopy.org.uk/pipermail/flimfit-users/2014-September/000005.html

For anything other than pure mono-exponential decays and  in order to maximise your SNR we strongly suggest measuring an irf
along with each experiment.

Some information about FLIMfit and irfs is available at https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough (about half-way
doen the page.)

All the best.

Ian





On 18 Sep 2014, at 12:48, Borst, Jan Willem <janwillem.borst at wur.nl<mailto:janwillem.borst at wur.nl>> wrote:

Dear all

We are trying to use FLIMfit 4.7.0 for analysing FLIM data.
We are able to load B-H data as well as PQ data (bin format) and obtain the intensity image.
Selection of a pixel displays the decay curve but not good fit is obtained.

Can someone maybe post guidelines which settings should be used?
There is FLIMfit documentation but that does not give a protocol of the steps to take.

All info is welcome.

Best

JW


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