<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<div><br>
</div>
<div>Hi Jan<br>
<br>
I simply meant that the SNR of the fitted lifetime is much lower using the tail-fitting approach as you discard a lot of the light.<br>
<br>
Would it be possible to see one of your data sets & associated irf ?<br>
<br>
Perhaps we could then work out what the problem is.<br>
<br>
Best <br>
<br>
Ian<br>
<br>
<br>
On 18 Sep 2014, at 13:36, Borst, Jan Willem <<a href="mailto:janwillem.borst@wur.nl">janwillem.borst@wur.nl</a>> wrote:<br>
<br>
<blockquote type="cite">Hi Ian<br>
<br>
We have IRF measured and have tried to use it and have tried it without.<br>
No fitting possible<br>
S/N is pretty good especially if you use bin option.<br>
<br>
Talk to you later<br>
<br>
JW<br>
On 18 Sep 2014, at 14:09, Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk">i.munro@imperial.ac.uk</a>> wrote:<br>
<br>
<blockquote type="cite">Dear Jan<br>
<br>
I guess the first question is “ do you have a measured irf ? “.<br>
If not , then you may , if your data is truly mono-exponential and there are enough photons,. be able to get a good lifetime by using the tail- fitting <br>
approach that I describe here:<br>
<br>
<a href="http://lists.openmicroscopy.org.uk/pipermail/flimfit-users/2014-September/000005.html">http://lists.openmicroscopy.org.uk/pipermail/flimfit-users/2014-September/000005.html</a><br>
<br>
For anything other than pure mono-exponential decays and in order to maximise your SNR we strongly suggest measuring an irf <br>
along with each experiment.<br>
<br>
Some information about FLIMfit and irfs is available at <a href="https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough">https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough</a> (about half-way <br>
doen the page.)<br>
<br>
All the best.<br>
<br>
Ian<br>
<br>
</blockquote>
</blockquote>
</div>
</body>
</html>