[ome-users] CZI: wrong number of timepoints

[Maier, Lorenz]

Dear Roger,

thanks for looking into this. The image was acquired with Zen black 2012, using the autosave function. Autosave was configured to split timepoints and positions (series).
All generated files together do have T=18 and S=140, but each individual file is only a C=3 Z=11 (T=1) subset of it.

Many of the other files are read correctly with T=1, but this is an example of the failing ones.

Best,
Lorenz

-----Ursprüngliche Nachricht-----
Von: ome-users-bounces at lists.openmicroscopy.org.uk [mailto:ome-users-bounces at lists.openmicroscopy.org.uk] Im Auftrag von Roger Leigh
Gesendet: Montag, 8. September 2014 14:55
An: ome-users at lists.openmicroscopy.org.uk
Betreff: Re: [ome-users] CZI: wrong number of timepoints

On 07/09/14 15:32, Maier, Lorenz wrote:

> even with the "open files individually" option of Fiji, I have a .czi 
> image that is reported to have 4 timepoints, but actually it has only one.

Dear Lorenz,

Thanks for uploading the image.  If you look at the image it's even stranger--the CZI reports T=18 and S=140, though this isn't reflected when you look at the dimensionality in Zen Blue.  Which version of Zen was used to acquire and save the image?  Did any postprocessing take place after acquisition e.g. reduction of the T and/or S dimensions?

I have opened a ticket for this, adding you as a CC:
   https://trac.openmicroscopy.org.uk/ome/ticket/12556


Thanks,
Roger

--
Dr Roger Leigh -- Open Microscopy Environment Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street,
Dundee DD1 5EH Scotland UK   Tel: (01382) 386364

The University of Dundee is a registered Scottish Charity, No: SC015096 _______________________________________________
ome-users mailing list
ome-users at lists.openmicroscopy.org.uk
http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users