[ome-users] CZI: wrong number of timepoints

Roger Leigh rleigh at dundee.ac.uk
Tue Sep 9 09:34:43 BST 2014


Dear Lorenz,

Thanks, I've added this additional information to the ticket.


Regards,
Roger

On 08/09/14 14:09, Maier, Lorenz wrote:
> Dear Roger,
>
> thanks for looking into this. The image was acquired with Zen black 2012, using the autosave function. Autosave was configured to split timepoints and positions (series).
> All generated files together do have T=18 and S=140, but each individual file is only a C=3 Z=11 (T=1) subset of it.
>
> Many of the other files are read correctly with T=1, but this is an example of the failing ones.
>
> Best,
> Lorenz
>
> -----Ursprüngliche Nachricht-----
> Von: ome-users-bounces at lists.openmicroscopy.org.uk [mailto:ome-users-bounces at lists.openmicroscopy.org.uk] Im Auftrag von Roger Leigh
> Gesendet: Montag, 8. September 2014 14:55
> An: ome-users at lists.openmicroscopy.org.uk
> Betreff: Re: [ome-users] CZI: wrong number of timepoints
>
> On 07/09/14 15:32, Maier, Lorenz wrote:
>
>> even with the "open files individually" option of Fiji, I have a .czi
>> image that is reported to have 4 timepoints, but actually it has only one.
>
> Dear Lorenz,
>
> Thanks for uploading the image.  If you look at the image it's even stranger--the CZI reports T=18 and S=140, though this isn't reflected when you look at the dimensionality in Zen Blue.  Which version of Zen was used to acquire and save the image?  Did any postprocessing take place after acquisition e.g. reduction of the T and/or S dimensions?
>
> I have opened a ticket for this, adding you as a CC:
>     https://trac.openmicroscopy.org.uk/ome/ticket/12556
>
>
> Thanks,
> Roger
>
> --
> Dr Roger Leigh -- Open Microscopy Environment Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street,
> Dundee DD1 5EH Scotland UK   Tel: (01382) 386364
>
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--
Dr Roger Leigh -- Open Microscopy Environment
Wellcome Trust Centre for Gene Regulation and Expression,
College of Life Sciences, University of Dundee, Dow Street,
Dundee DD1 5EH Scotland UK   Tel: (01382) 386364

The University of Dundee is a registered Scottish Charity, No: SC015096



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