[ome-users] Mirror before stitching?

[Curtis Rueden]

Hi Sam,

We recently added an "invert Y axis coordinates" option (and one for X as
well) to the Stitching plugins. Did you try that?

The Fiji Grid/Collection Stitching plugin, in case you haven't tried it,
uses Bio-Formats and is much more powerful than the BF Importer's basic
stitching feature.

Regards,
Curtis
 On Jun 16, 2013 12:01 PM, "Sam Lord" <sjlord at berkeley.edu> wrote:

> Importing stacks from Micro-Manager works well using Bio-Formats Importer,
> and the stacks option seems to read location directly from metadata. The
> only issue I have is that my camera must be rotated 180 degrees, because
> the columns stitch together well, but each row is upside-down.
>
> I tried importing the stack, flipping, exporting, then reimorting, but
> something must have gotten lost in the metadata in that maneuver.
>
> Any chance there is a simple way to tell the stitching that the images
> are flipped?
>
> -Sam
>
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