[ome-users] Mirror before stitching?

[Sam Lord]

I tried that just once, and it seemed to add black bars between each row. I'll experiment with it more next week. Thanks for the suggestion. 

-Sam

On Sun, Jun 16, 2013 at 1:49 PM, Curtis Rueden <ctrueden at wisc.edu> wrote:

> Hi Sam,
> We recently added an "invert Y axis coordinates" option (and one for X as
> well) to the Stitching plugins. Did you try that?
> The Fiji Grid/Collection Stitching plugin, in case you haven't tried it,
> uses Bio-Formats and is much more powerful than the BF Importer's basic
> stitching feature.
> Regards,
> Curtis
>  On Jun 16, 2013 12:01 PM, "Sam Lord" <sjlord at berkeley.edu> wrote:
>> Importing stacks from Micro-Manager works well using Bio-Formats Importer,
>> and the stacks option seems to read location directly from metadata. The
>> only issue I have is that my camera must be rotated 180 degrees, because
>> the columns stitch together well, but each row is upside-down.
>>
>> I tried importing the stack, flipping, exporting, then reimorting, but
>> something must have gotten lost in the metadata in that maneuver.
>>
>> Any chance there is a simple way to tell the stitching that the images
>> are flipped?
>>
>> -Sam
>>
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>>
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