[ome-users] Bioformats only imports parts of .nd2 file

[Anna-Lena Merten]

Hi everyone,

using the NIS Elements software we recorded stacks of images (time 
lapse, interval: 100ms, duration: 2 min, Loops: 1201; 1 position, 1 
channel) which resulted in .nd2-files bigger than 7GB.

Bioformats 5.9.1 does not open the complete stack, there are obviously 
images missing from the end of the stack (NIS Elements still opens the 
whole time lapse). Even though the files are bigger than 7GB, ImageJ 
only indicates 2GB but does not respond with an error message 
(screenshots in appendix). There do not seem to be problems with files 
smaller than 2GB.

I am working with Windows 10, ImageJ 1.52e and Java 1.8.0_66 (64-bit).

Thank you for your help!

Best regards,

Anna-Lena

-- 
Anna-Lena Merten

Institute of Medical Biotechnology
Friedrich-Alexander-University Erlangen-Nuremberg
Ulrich-Schalk-Str.3, 91056 Erlangen, Germany

phone: +49 9131 85 69643
FAX: +49 9131 85 23002
web: http://www.mbt.tf.uni-erlangen.de/

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