[ome-users] Issue when reading czi lines scans

[Ponti Aaron]

Hi,

I attach two small line-scan z stacks Image1.czi and Image2.czi with following characteristics:

Image1 (read properly):
Two channels (green and red)
Actual Dimensions: x y z =512 1 80
Pixel size: x z= 0.208um 0.5um

Image2 (read incorrectly):
One channels (only red)
Actual Dimensions: x,y,z =512, 1, 240
Pixel size: x,z= 0.208um, 0.5um

Image1 can be read properly by bio-formats 5.1.8 (resulting in a 512 x 1 x 80 dataset). Image2.czi is read incorrectly, resulting in a 512 x 1 x 1 dataset instead of 512 x 1 x 240 (the z information is lost). The only obvious difference between the two files is the number of channels.

Thanks for looking into this.
a2

----
Dr. Aaron Ponti
Software and Data Management Engineer
Image Analysis Specialist
Single Cell Unit
Department of Biosystems Science and Engineering (D-BSSE)
ETH Zürich
Office 2.24
Mattenstrasse 26
4058 Basel, Switzerland
aaron.ponti at bsse.ethz.ch
+41 61 387 33 74
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