<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;">
<div>Hi,</div>
<div><br>
</div>
<div>I attach two small line-scan z stacks Image1.czi and Image2.czi with following characteristics:</div>
<div><br>
</div>
<div>
<div>Image1 (read properly):</div>
<div>Two channels (green and red)</div>
<div>Actual Dimensions: x y z =512 1 80</div>
<div>Pixel size: x z= 0.208um 0.5um</div>
<div><br>
</div>
<div>
<div>Image2 (read incorrectly):</div>
<div>One channels (only red)</div>
<div>Actual Dimensions: x,y,z =512, 1, 240</div>
<div>Pixel size: x,z= 0.208um, 0.5um</div>
</div>
</div>
<div><br>
</div>
<div>
<div>Image1 can be read properly by bio-formats 5.1.8 (resulting in a 512 x 1 x 80 dataset). Image2.czi is read incorrectly, resulting in a 512 x 1 x 1 dataset instead of 512 x 1 x 240 (the z information is lost). The only obvious difference between the two
files is the number of channels.</div>
</div>
<div><br>
</div>
<div>Thanks for looking into this.</div>
<div>a2</div>
<div><br>
</div>
<div>
<div id="MAC_OUTLOOK_SIGNATURE">
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">----</font></font></div>
<div>
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">Dr. Aaron Ponti</font></font></div>
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">Software and Data Management Engineer</font></font></div>
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">Image Analysis Specialist</font></font></div>
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">Single Cell Unit</font></font></div>
<div style="font-family: Calibri, sans-serif; "><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri">Department of Biosystems Science and Engineering (D-BSSE)</font></font></div>
<div style="font-family: Calibri, sans-serif; "><span class="Apple-style-span" style="font-family: Calibri; ">ETH Zürich<br>
</span></div>
<div style="font-family: Calibri, sans-serif; "><span class="Apple-style-span" style="font-family: Calibri; ">Office 2.24</span><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri"><span class="Apple-style-span" style="font-size: 14px; "><br>
Mattenstrasse 26<br>
4058 Basel, </span></font></font><span class="Apple-style-span" style="font-family: Calibri; ">Switzerland</span></div>
<div style="font-family: Calibri, sans-serif; ">aaron.ponti@bsse.ethz.ch</div>
<div style="font-family: Calibri, sans-serif; ">
<div><font class="Apple-style-span" color="rgb(0, 0, 0)"><font class="Apple-style-span" face="Calibri"><span class="Apple-style-span" style="font-size: 14px; ">+41 </span></font></font>61 387 33 74</div>
</div>
</div>
</div>
</div>
</body>
</html>