[ome-users] FW: Strange issue when bioformats_package.jar is used instead of loci_tools.jar
Ponti Aaron
aaron.ponti at bsse.ethz.ch
Thu Sep 11 08:14:39 BST 2014
Dear list,
I am using bioformats in the openBIS Importer Toolset (http://www.scs2.net/next/index.php?id=150) to extract metadata information from microscopy files and I am facing an issue that puzzles me. Consider the following code snippet (I only leave what is important):
<code>
...
// Create the reader
reader = new ImageProcessorReader(
new ChannelSeparator(
LociPrefs.makeImageReader()));
...
try {
factory = new ServiceFactory();
service = factory.getInstance(OMEXMLService.class);
} catch (DependencyException e) {
...
}
try {
omexmlMeta = service.createOMEXMLMetadata();
} catch (ServiceException e) {
...
}
reader.setMetadataStore(omexmlMeta);
// Try to open the image file
try {
reader.setId(filename.getCanonicalPath());
} catch (FormatException e) {
...
}
</code>
Now, if I add loci_tools.jar (5.0.4) to the build path, everything works without any problem; if I add bioformats_package.jar (5.0.4, both downloaded from http://downloads.openmicroscopy.org/bio-formats/5.0.4/) I get the following when
reader.setId(filename.getCanonicalPath());
is reached:
<log>
14:22:53.768 [SwingWorker-pool-2-thread-5] INFO loci.formats.ImageReader - ND2Reader initializing D:\user\pontia\Experiment_2\Captured for 4.nd2
14:22:53.768 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler - ND2Reader initializing D:\user\pontia\Experiment_2\Captured for 4.nd2
14:22:53.768 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler - loci.formats.in.ND2Reader.initFile(D:\user\pontia\Experiment_2\Captured for 4.nd2)
14:22:53.768 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler - NativeND2Reader initializing D:\user\pontia\Experiment_2\Captured for 4.nd2
14:22:53.768 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler - loci.formats.in.NativeND2Reader.initFile(D:\user\pontia\Experiment_2\Captured for 4.nd2)
14:22:53.768 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Searching for blocks
14:22:53.768 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ND2 FILE SIG' 0%
14:22:53.774 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ImageCalibra' 0%
14:22:53.782 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler -
java.lang.NegativeArraySizeException: null
at loci.common.RandomAccessInputStream.readString(RandomAccessInputStream.java:406) ~[bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1570) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.initFile(NativeND2Reader.java:420) [bioformats_package.jar:na]
at loci.formats.FormatReader.setId(FormatReader.java:1317) [bioformats_package.jar:na]
at loci.formats.DelegateReader.setId(DelegateReader.java:253) [bioformats_package.jar:na]
at loci.formats.ImageReader.setId(ImageReader.java:753) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:270) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.init(MicroscopyReader.java:106) [bin/:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.<init>(MicroscopyReader.java:66) [bin/:na]
at ch.ethz.scu.obit.microscopy.processors.data.MicroscopyProcessor$MicroscopyFile.scanForSeries(MicroscopyProcessor.java:524) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:371) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:1) [bin/:na]
at javax.swing.SwingWorker$1.call(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.FutureTask.run(Unknown Source) [na:1.7.0_51]
at javax.swing.SwingWorker.run(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor.runWorker(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor$Worker.run(Unknown Source) [na:1.7.0_51]
at java.lang.Thread.run(Unknown Source) [na:1.7.0_51]
14:22:53.795 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler -
java.lang.NegativeArraySizeException: null
at loci.common.RandomAccessInputStream.readString(RandomAccessInputStream.java:406) ~[bioformats_package.jar:na]
...
14:22:53.990 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler -
java.lang.NegativeArraySizeException: null
at loci.common.RandomAccessInputStream.readString(RandomAccessInputStream.java:406) ~[bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1570) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.iterateIn(NativeND2Reader.java:1644) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.initFile(NativeND2Reader.java:420) [bioformats_package.jar:na]
at loci.formats.FormatReader.setId(FormatReader.java:1317) [bioformats_package.jar:na]
at loci.formats.DelegateReader.setId(DelegateReader.java:253) [bioformats_package.jar:na]
at loci.formats.ImageReader.setId(ImageReader.java:753) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:270) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.init(MicroscopyReader.java:106) [bin/:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.<init>(MicroscopyReader.java:66) [bin/:na]
at ch.ethz.scu.obit.microscopy.processors.data.MicroscopyProcessor$MicroscopyFile.scanForSeries(MicroscopyProcessor.java:524) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:371) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:1) [bin/:na]
at javax.swing.SwingWorker$1.call(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.FutureTask.run(Unknown Source) [na:1.7.0_51]
at javax.swing.SwingWorker.run(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor.runWorker(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor$Worker.run(Unknown Source) [na:1.7.0_51]
at java.lang.Thread.run(Unknown Source) [na:1.7.0_51]
14:22:54.005 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ImageMetadat' 0%
14:22:54.008 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ImageDataSeq' 2%
14:22:54.008 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ImageTextInf' 94%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 95%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 95%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 95%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 95%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.009 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|C' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|P' 96%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|P' 97%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|X' 97%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|Y' 97%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|Z' 97%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|Z' 97%
14:22:54.010 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|Z' 97%
14:22:54.011 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 97%
14:22:54.011 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 98%
14:22:54.011 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 98%
14:22:54.012 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 98%
14:22:54.012 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomDataVa' 98%
14:22:54.013 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|A' 99%
14:22:54.013 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|A' 99%
14:22:54.013 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'CustomData|A' 99%
14:22:54.013 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ImageAttribu' 99%
14:22:54.013 [SwingWorker-pool-2-thread-5] INFO loci.formats.FormatHandler - Parsing block 'ND2 FILEMAP ' 99%
14:22:54.015 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatHandler - Could not parse XML
java.io.IOException: null
at loci.common.xml.XMLTools.parseXML(XMLTools.java:436) ~[bioformats_package.jar:na]
at loci.common.xml.XMLTools.parseXML(XMLTools.java:414) ~[bioformats_package.jar:na]
at loci.common.xml.XMLTools.parseXML(XMLTools.java:393) ~[bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.parseText(NativeND2Reader.java:2054) [bioformats_package.jar:na]
at loci.formats.in.NativeND2Reader.initFile(NativeND2Reader.java:726) [bioformats_package.jar:na]
at loci.formats.FormatReader.setId(FormatReader.java:1317) [bioformats_package.jar:na]
at loci.formats.DelegateReader.setId(DelegateReader.java:253) [bioformats_package.jar:na]
at loci.formats.ImageReader.setId(ImageReader.java:753) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:270) [bioformats_package.jar:na]
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:569) [bioformats_package.jar:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.init(MicroscopyReader.java:106) [bin/:na]
at ch.ethz.scu.obit.microscopy.readers.MicroscopyReader.<init>(MicroscopyReader.java:66) [bin/:na]
at ch.ethz.scu.obit.microscopy.processors.data.MicroscopyProcessor$MicroscopyFile.scanForSeries(MicroscopyProcessor.java:524) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:371) [bin/:na]
at ch.ethz.scu.obit.microscopy.gui.viewers.data.MicroscopyViewer$1Worker.doInBackground(MicroscopyViewer.java:1) [bin/:na]
at javax.swing.SwingWorker$1.call(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.FutureTask.run(Unknown Source) [na:1.7.0_51]
at javax.swing.SwingWorker.run(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor.runWorker(Unknown Source) [na:1.7.0_51]
at java.util.concurrent.ThreadPoolExecutor$Worker.run(Unknown Source) [na:1.7.0_51]
at java.lang.Thread.run(Unknown Source) [na:1.7.0_51]
Caused by: org.xml.sax.SAXParseException: Content is not allowed in prolog.
at com.sun.org.apache.xerces.internal.util.ErrorHandlerWrapper.createSAXParseException(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.util.ErrorHandlerWrapper.fatalError(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLErrorReporter.reportError(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLErrorReporter.reportError(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLScanner.reportFatalError(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLDocumentScannerImpl$PrologDriver.next(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLDocumentScannerImpl.next(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.impl.XMLDocumentFragmentScannerImpl.scanDocument(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.parsers.XML11Configuration.parse(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.parsers.XML11Configuration.parse(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.parsers.XMLParser.parse(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.parsers.AbstractSAXParser.parse(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.jaxp.SAXParserImpl$JAXPSAXParser.parse(Unknown Source) ~[na:1.7.0_51]
at com.sun.org.apache.xerces.internal.jaxp.SAXParserImpl.parse(Unknown Source) ~[na:1.7.0_51]
at javax.xml.parsers.SAXParser.parse(Unknown Source) ~[na:1.7.0_51]
at loci.common.xml.XMLTools.parseXML(XMLTools.java:428) ~[bioformats_package.jar:na]
... 21 common frames omitted
14:22:54.029 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatTools - Expected positive value for PhysicalSizeZ; got 0.0
14:22:54.029 [SwingWorker-pool-2-thread-5] DEBUG loci.formats.FormatTools - Expected positive value for EmissionWavelength; got 0
14:22:54.037 [SwingWorker-pool-2-thread-5] DEBUG loci.common.Location - Location.mapFile: embedded-stream.raw -> null
</log>
There is absolutely no issue (logged at least) when loci_tools.jar is used. Interestingly, the metadata seems to be read even when bioformats_package.jar is used, despite all exceptions. The problem is that openBIS monitors the execution of dropboxes and if it catches exceptions it stops the registration of data. This is the case when bioformats_package.jar is used, and is not when loci_tools.jar is used.
I attached the full log and the ND2 file from the example (other ND2 files behave the same; LSM files, for instance, don't). Can anyone give me a hint what might be happening here? Nothing seems to be missing from the bioformats_package.jar that would be in loci_tools.jar.
Thanks a lot,
a2
----
Dr. Aaron Ponti
Software and Data Management Engineer
Single Cell Unit
Department of Biosystems Science and Engineering (D-BSSE)
ETH Zürich
Office 2.24
Mattenstrasse 26
4058 Basel, Switzerland
aaron.ponti at bsse.ethz.ch<mailto:aaron.ponti at bsse.ethz.ch>
+41 61 387 33 74
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