[ome-users] trouble opening .nd2 files

[Melissa Linkert]

Hi Pradeep,

> I am trying to open .nd2 files in Bisque and am encountering following
> problems
> My two channel images (DIC and DAPI) are defaulting to green and red false
> colors with no option of changing the colors.
> My deconvolved images are not displayed correctly. Please see attached
> screenshots.
> 
> The images were collected on a Nikon T1 Eclipse running NIS Elements 4.
> Versions 4.10.01 Build 834

Which version of Bio-Formats are you using?  If you are using something
prior to the current stable version (4.4.8), then I would suggest first
upgrading to see if that fixes the problem.

If upgrading to 4.4.8 does not solve the problem, then it would be very
helpful if you could send one of the original .nd2 files.  Files can be
uploaded to http://qa.openmicroscopy.org.uk/qa/upload/

Regards,
-Melissa

On Mon, Sep 16, 2013 at 10:00:56AM -0700, Pradeep Joshi wrote:
> I am trying to open .nd2 files in Bisque and am encountering following
> problems
> My two channel images (DIC and DAPI) are defaulting to green and red false
> colors with no option of changing the colors.
> My deconvolved images are not displayed correctly. Please see attached
> screenshots.
> 
> The images were collected on a Nikon T1 Eclipse running NIS Elements 4.
> Versions 4.10.01 Build 834
> 
> Please help.
> 
> Best
> Pradeep
> 
> "The known is finite, the unknown infinite. Intellectually we stand on an
> islet in the midst of an illimitable ocean of inexplicability. Our business
> every generation is to reclaim a little more land"- T.H. Huxley
> 
> Dr. Pradeep M. Joshi
> Neuroscience Research Institute
> University of California Santa Barbara
> Santa Barbara, CA 93106
> 
> Lab: 805 893 8090
> Cell: 805 680 1369


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