[ome-users] Channel colours

[Josh Moore]

On Nov 7, 2013, at 10:01 AM, Paul Thomas (BIO) wrote:

> Dear Melissa,

Paul,

> Thank you for looking into this problem. Yes, please could you let us know any progress on resolution of the bug? Please could you also add my colleague (Wouter Hendrickx - cced in this message) to the ome-users list?

Done and Done

Regards,
~Josh

> Best wishes,
> Paul.
> ____________________
> Dr. Paul Thomas, Director,
> The Henry Wellcome Laboratory for Cell Imaging,
> School of Biological Sciences,
> University of East Anglia,
> Norwich Research Park,
> Norwich,
> NR4 7TJ,
> United Kingdom.
> 
> e-mail: p.thomas at uea.ac.uk
> Tel: +44-1603-592196
> Fax: +44-1603-592250
> Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
> Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
> 
>> -----Original Message-----
>> From: Melissa Linkert [mailto:melissa.linkert at gmail.com] On Behalf Of
>> Melissa Linkert
>> Sent: 07 November 2013 01:18
>> To: Paul Thomas (BIO)
>> Cc: ome-users at lists.openmicroscopy.org.uk
>> Subject: Re: [ome-users] Channel colours
>> 
>> Hi Paul,
>> 
>>> I'm having an issue retaining my colour setting in my ome-tiffs
>>> 
>>> Images are acquired with axiovision 3 channels blue , red , green.
>> Opened in Fiji with bioformats as separate images so I can use the
>> image calculator and remove background, then I create a stack from the
>> 3 open images blue red and green, and convert the stack to a hyperstack
>> using 3 channels without any z stack or t settings. Finally I export to
>> ome-tiff using bioformats. If I then close the image and reopen, the
>> colours are lost and all channels are red.
>> 
>> Thank you very much for the bug report.  I can reproduce the problem,
>> and have filed a ticket to fix it on our issue tracking system:
>> 
>> http://trac.openmicroscopy.org.uk/ome/ticket/11658
>> 
>> If you would like to be automatically notified of updates to that
>> ticket, please let me know and I'll CC you.
>> 
>> Regards,
>> -Melissa
>> 
>> On Mon, Nov 04, 2013 at 04:43:04PM +0000, Paul Thomas (BIO) wrote:
>>> Dear  all
>>> 
>>> I'm having an issue retaining my colour setting in my ome-tiffs
>>> 
>>> Images are acquired with axiovision 3 channels blue , red , green.
>> Opened in Fiji with bioformats as separate images so I can use the
>> image calculator and remove background, then I create a stack from the
>> 3 open images blue red and green, and convert the stack to a hyperstack
>> using 3 channels without any z stack or t settings. Finally I export to
>> ome-tiff using bioformats. If I then close the image and reopen, the
>> colours are lost and all channels are red.
>>> 
>>> Many thanks,
>>> 
>>> Paul.
>>> 
>>> ____________________
>>> Dr. Paul Thomas, Director,
>>> The Henry Wellcome Laboratory for Cell Imaging, School of Biological
>>> Sciences, University of East Anglia, Norwich Research Park, Norwich,
>>> NR4 7TJ,
>>> United Kingdom.
>>> 
>>> e-mail: p.thomas at uea.ac.uk
>>> Tel: +44-1603-592196
>>> Fax: +44-1603-592250
>>> Imaging web-site:
>>> http://www.uea.ac.uk/biological-sciences/research/facilities/henry-
>> wel
>>> lcome-lab Personal web-page:
>>> http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
>>> 
>> 
>>> _______________________________________________
>>> ome-users mailing list
>>> ome-users at lists.openmicroscopy.org.uk
>>> http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users
> 
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