[ome-users] Channel colours

[Paul Thomas (BIO)]

Dear Melissa,

Thank you for looking into this problem. Yes, please could you let us know any progress on resolution of the bug? Please could you also add my colleague (Wouter Hendrickx - cced in this message) to the ome-users list?

Best wishes,
Paul.
____________________
Dr. Paul Thomas, Director,
The Henry Wellcome Laboratory for Cell Imaging,
School of Biological Sciences,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.

e-mail: p.thomas at uea.ac.uk
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548

> -----Original Message-----
> From: Melissa Linkert [mailto:melissa.linkert at gmail.com] On Behalf Of
> Melissa Linkert
> Sent: 07 November 2013 01:18
> To: Paul Thomas (BIO)
> Cc: ome-users at lists.openmicroscopy.org.uk
> Subject: Re: [ome-users] Channel colours
> 
> Hi Paul,
> 
> > I'm having an issue retaining my colour setting in my ome-tiffs
> >
> > Images are acquired with axiovision 3 channels blue , red , green.
> Opened in Fiji with bioformats as separate images so I can use the
> image calculator and remove background, then I create a stack from the
> 3 open images blue red and green, and convert the stack to a hyperstack
> using 3 channels without any z stack or t settings. Finally I export to
> ome-tiff using bioformats. If I then close the image and reopen, the
> colours are lost and all channels are red.
> 
> Thank you very much for the bug report.  I can reproduce the problem,
> and have filed a ticket to fix it on our issue tracking system:
> 
> http://trac.openmicroscopy.org.uk/ome/ticket/11658
> 
> If you would like to be automatically notified of updates to that
> ticket, please let me know and I'll CC you.
> 
> Regards,
> -Melissa
> 
> On Mon, Nov 04, 2013 at 04:43:04PM +0000, Paul Thomas (BIO) wrote:
> > Dear  all
> >
> > I'm having an issue retaining my colour setting in my ome-tiffs
> >
> > Images are acquired with axiovision 3 channels blue , red , green.
> Opened in Fiji with bioformats as separate images so I can use the
> image calculator and remove background, then I create a stack from the
> 3 open images blue red and green, and convert the stack to a hyperstack
> using 3 channels without any z stack or t settings. Finally I export to
> ome-tiff using bioformats. If I then close the image and reopen, the
> colours are lost and all channels are red.
> >
> > Many thanks,
> >
> > Paul.
> >
> > ____________________
> > Dr. Paul Thomas, Director,
> > The Henry Wellcome Laboratory for Cell Imaging, School of Biological
> > Sciences, University of East Anglia, Norwich Research Park, Norwich,
> > NR4 7TJ,
> > United Kingdom.
> >
> > e-mail: p.thomas at uea.ac.uk
> > Tel: +44-1603-592196
> > Fax: +44-1603-592250
> > Imaging web-site:
> > http://www.uea.ac.uk/biological-sciences/research/facilities/henry-
> wel
> > lcome-lab Personal web-page:
> > http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
> >
> 
> > _______________________________________________
> > ome-users mailing list
> > ome-users at lists.openmicroscopy.org.uk
> > http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users