[ome-users] Channel colours

[Paul Thomas (BIO)]

Josh,

Many thanks for the quick bug-fix.

Paul
____________________
Dr. Paul Thomas, Director,
The Henry Wellcome Laboratory for Cell Imaging,
School of Biological Sciences,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.

e-mail: p.thomas at uea.ac.uk
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548


> -----Original Message-----
> From: Josh Moore [mailto:josh at glencoesoftware.com]
> Sent: 07 November 2013 09:07
> To: Paul Thomas (BIO)
> Cc: melissa at glencoesoftware.com; ome-users at lists.openmicroscopy.org.uk;
> Wouter Hendrickx (MED)
> Subject: Re: [ome-users] Channel colours
> 
> 
> On Nov 7, 2013, at 10:01 AM, Paul Thomas (BIO) wrote:
> 
> > Dear Melissa,
> 
> Paul,
> 
> > Thank you for looking into this problem. Yes, please could you let us
> know any progress on resolution of the bug? Please could you also add
> my colleague (Wouter Hendrickx - cced in this message) to the ome-users
> list?
> 
> Done and Done
> 
> Regards,
> ~Josh
> 
> > Best wishes,
> > Paul.
> > ____________________
> > Dr. Paul Thomas, Director,
> > The Henry Wellcome Laboratory for Cell Imaging, School of Biological
> > Sciences, University of East Anglia, Norwich Research Park, Norwich,
> > NR4 7TJ,
> > United Kingdom.
> >
> > e-mail: p.thomas at uea.ac.uk
> > Tel: +44-1603-592196
> > Fax: +44-1603-592250
> > Imaging web-site:
> > http://www.uea.ac.uk/biological-sciences/research/facilities/henry-
> wel
> > lcome-lab Personal web-page:
> > http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
> >
> >> -----Original Message-----
> >> From: Melissa Linkert [mailto:melissa.linkert at gmail.com] On Behalf
> Of
> >> Melissa Linkert
> >> Sent: 07 November 2013 01:18
> >> To: Paul Thomas (BIO)
> >> Cc: ome-users at lists.openmicroscopy.org.uk
> >> Subject: Re: [ome-users] Channel colours
> >>
> >> Hi Paul,
> >>
> >>> I'm having an issue retaining my colour setting in my ome-tiffs
> >>>
> >>> Images are acquired with axiovision 3 channels blue , red , green.
> >> Opened in Fiji with bioformats as separate images so I can use the
> >> image calculator and remove background, then I create a stack from
> >> the
> >> 3 open images blue red and green, and convert the stack to a
> >> hyperstack using 3 channels without any z stack or t settings.
> >> Finally I export to ome-tiff using bioformats. If I then close the
> >> image and reopen, the colours are lost and all channels are red.
> >>
> >> Thank you very much for the bug report.  I can reproduce the
> problem,
> >> and have filed a ticket to fix it on our issue tracking system:
> >>
> >> http://trac.openmicroscopy.org.uk/ome/ticket/11658
> >>
> >> If you would like to be automatically notified of updates to that
> >> ticket, please let me know and I'll CC you.
> >>
> >> Regards,
> >> -Melissa
> >>
> >> On Mon, Nov 04, 2013 at 04:43:04PM +0000, Paul Thomas (BIO) wrote:
> >>> Dear  all
> >>>
> >>> I'm having an issue retaining my colour setting in my ome-tiffs
> >>>
> >>> Images are acquired with axiovision 3 channels blue , red , green.
> >> Opened in Fiji with bioformats as separate images so I can use the
> >> image calculator and remove background, then I create a stack from
> >> the
> >> 3 open images blue red and green, and convert the stack to a
> >> hyperstack using 3 channels without any z stack or t settings.
> >> Finally I export to ome-tiff using bioformats. If I then close the
> >> image and reopen, the colours are lost and all channels are red.
> >>>
> >>> Many thanks,
> >>>
> >>> Paul.
> >>>
> >>> ____________________
> >>> Dr. Paul Thomas, Director,
> >>> The Henry Wellcome Laboratory for Cell Imaging, School of
> Biological
> >>> Sciences, University of East Anglia, Norwich Research Park,
> Norwich,
> >>> NR4 7TJ,
> >>> United Kingdom.
> >>>
> >>> e-mail: p.thomas at uea.ac.uk
> >>> Tel: +44-1603-592196
> >>> Fax: +44-1603-592250
> >>> Imaging web-site:
> >>> http://www.uea.ac.uk/biological-sciences/research/facilities/henry-
> >> wel
> >>> lcome-lab Personal web-page:
> >>> http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
> >>>
> >>
> >>> _______________________________________________
> >>> ome-users mailing list
> >>> ome-users at lists.openmicroscopy.org.uk
> >>> http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users
> >
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