[ome-users] Channel colours

[Melissa Linkert]

Hi Paul,

> I'm having an issue retaining my colour setting in my ome-tiffs
> 
> Images are acquired with axiovision 3 channels blue , red , green. Opened in Fiji with bioformats as separate images so I can use the image calculator and remove background, then I create a stack from the 3 open images blue red and green, and convert the stack to a hyperstack using 3 channels without any z stack or t settings. Finally I export to ome-tiff using bioformats. If I then close the image and reopen, the colours are lost and all channels are red.

Thank you very much for the bug report.  I can reproduce the problem, and have
filed a ticket to fix it on our issue tracking system:

http://trac.openmicroscopy.org.uk/ome/ticket/11658

If you would like to be automatically notified of updates to that
ticket, please let me know and I'll CC you.

Regards,
-Melissa

On Mon, Nov 04, 2013 at 04:43:04PM +0000, Paul Thomas (BIO) wrote:
> Dear  all
> 
> I'm having an issue retaining my colour setting in my ome-tiffs
> 
> Images are acquired with axiovision 3 channels blue , red , green. Opened in Fiji with bioformats as separate images so I can use the image calculator and remove background, then I create a stack from the 3 open images blue red and green, and convert the stack to a hyperstack using 3 channels without any z stack or t settings. Finally I export to ome-tiff using bioformats. If I then close the image and reopen, the colours are lost and all channels are red.
> 
> Many thanks,
> 
> Paul.
> 
> ____________________
> Dr. Paul Thomas, Director,
> The Henry Wellcome Laboratory for Cell Imaging,
> School of Biological Sciences,
> University of East Anglia,
> Norwich Research Park,
> Norwich,
> NR4 7TJ,
> United Kingdom.
> 
> e-mail: p.thomas at uea.ac.uk
> Tel: +44-1603-592196
> Fax: +44-1603-592250
> Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
> Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548
> 

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