[ome-users] Channel colours

[Paul Thomas (BIO)]

Dear  all

I'm having an issue retaining my colour setting in my ome-tiffs

Images are acquired with axiovision 3 channels blue , red , green. Opened in Fiji with bioformats as separate images so I can use the image calculator and remove background, then I create a stack from the 3 open images blue red and green, and convert the stack to a hyperstack using 3 channels without any z stack or t settings. Finally I export to ome-tiff using bioformats. If I then close the image and reopen, the colours are lost and all channels are red.

Many thanks,

Paul.

____________________
Dr. Paul Thomas, Director,
The Henry Wellcome Laboratory for Cell Imaging,
School of Biological Sciences,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.

e-mail: p.thomas at uea.ac.uk
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548

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