[ome-users] MetaMorph nd files with repeated wavelengths

[Ben Timney]

Hi,

I have a problem opening MetaMorph .nd files if two channels used the same wavelength. In my case, two FITC channels with different exposure settings were collected at each Z-T. Bioformats creates the right number of image planes for the correct number of channels, but only one of the FITC channels has image data. The images in the other channel just have all zero pixels. I assume the repeated channel names are confusing to the software.

Problem seen when opening such files in MatLab using BioFormats 4.4.4, (also reproduced in ImageJ).

DropBox link to example data

Ben Timney

________________
Ben Timney, PhD
Research Associate
Rout Laboratory,
1230 York Ave, #213
New York, NY 10065

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