[ome-users] Andor tif data to OME-tif conversion

[Juergen Helmers]

Hi,

I have image data from a screen using an Andor IQ2 instrument. The image
data seems to be 16-bit and can be imported into Omero resulting in images
with the correct number of channels (here two, red and green).

BUT:

The image are part of a screen of a 96 well plate. per well there are 9
ROI, each stack of multi-channel image files. What I would like to do is
import the images as a screen, resulting in a plate of images incorporating
the x,y information to place the images inside the grid of the 96 well
plate. In addition I have setup a rails application converting CSV
annoation sfile to a postgres database and I would like to add the
annoation to the metadata before input.

this was the plan:

convert Andor tif file to OME-tif file adding the screen > plate >
acquisition > well > ROI information to the header

When I try to rewrite the Exif information of any Andor file it results in
a broken file:

exiv2 -pa test.tif > header_info.txt
convert -comment "@header_info.txt" test.tif test_new.tif

When I import the new tif file although the extracted and rewritten header
information are identical the file when imported into Omero is a black and
white single channel image. I have used this method and used software
before to rewrite molecular devices files to create OME-tif files.

Has anyone ever dealt with Andor images in order to make them part of a
screen, or has some tips on which software to use to rewrite the tif header
of Andor files?

Cheers
Juergen


-- 
*Dr. Juergen Helmers*
*Chief Developer* | webnow | http://www.web-now.de
*email:*juergen.helmers at gmail.com | *tel:*+49 30 37301306 | *skype:*helmerj
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.openmicroscopy.org.uk/pipermail/ome-users/attachments/20120308/4a5fba07/attachment.html>