Hi,<br><br>I have image data from a screen using an Andor IQ2 instrument. The image data seems to be 16-bit and can be imported into Omero resulting in images with the correct number of channels (here two, red and green). <br>
<br>BUT:<br><br>The image are part of a screen of a 96 well plate. per well there are 9 ROI, each stack of multi-channel image files. What I would like to do is import the images as a screen, resulting in a plate of images incorporating the x,y information to place the images inside the grid of the 96 well plate. In addition I have setup a rails application converting CSV annoation sfile to a postgres database and I would like to add the annoation to the metadata before input. <br>
<br>this was the plan:<br><br>convert Andor tif file to OME-tif file adding the screen > plate > acquisition > well > ROI information to the header<br><br>When I try to rewrite the Exif information of any Andor file it results in a broken file:<br>
<br>exiv2 -pa test.tif > header_info.txt<br>convert -comment "@header_info.txt" test.tif test_new.tif<br><br>When I import the new tif file although the extracted and rewritten header information are identical the file when imported into Omero is a black and white single channel image. I have used this method and used software before to rewrite molecular devices files to create OME-tif files.<br>
<br>Has anyone ever dealt with Andor images in order to make them part of a screen, or has some tips on which software to use to rewrite the tif header of Andor files?<br><br>Cheers<br>Juergen<br><br clear="all"><br>-- <br>
<font><font color="#333333"><b>Dr. Juergen Helmers</b></font><br>
<i>Chief Developer</i> | webnow | <font color="#3366ff"><a href="http://www.web-now.de/" target="_blank">http://www.web-now.de</a></font><br>
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