[ome-users] BioFormat problem with some BioRad .1sc files

Melissa Linkert melissa at glencoesoftware.com
Wed Apr 25 20:35:59 BST 2012


Hi Geert,

> I noticed a problem with the Bio-Formats library while reading
> BioRad .1sc files (for the GelDoc system).
> 
> Some files are misinterpreted and show the wrong result. Most files
> however are correct.
> 
> This is the setting :
> 
> - BioFormat "Trunk build" from 20 april 2012
> - ImageJ 1.46j (Fiji distribution)
> - Java 1.6.0_24 (32 bit)
> - Windows XP (32 bit)
> 
> All files in full resolution can be found in this zip file =
> 
> http://www.MolecularBiotechnology.UGent.be/files/biorad-1sc.zip

Thank you very much for reporting this problem, and for providing all of
the information necessary to duplicate and fix it.  We have just now
implemented a fix, so the upcoming 4.4 release of Bio-Formats should
open both .1sc files correctly.

Regards,
-Melissa

On Mon, Apr 23, 2012 at 03:18:08PM +0200, Geert Meesen wrote:
> Hi all,
> 
> I noticed a problem with the Bio-Formats library while reading
> BioRad .1sc files (for the GelDoc system).
> 
> Some files are misinterpreted and show the wrong result. Most files
> however are correct.
> 
> This is the setting :
> 
> - BioFormat "Trunk build" from 20 april 2012
> - ImageJ 1.46j (Fiji distribution)
> - Java 1.6.0_24 (32 bit)
> - Windows XP (32 bit)
> 
> All files in full resolution can be found in this zip file =
> 
> http://www.MolecularBiotechnology.UGent.be/files/biorad-1sc.zip
> 
> It contains the following files :
> 
> - good example1.1sc = the original .1sc file showing an example that
> imports ok in Bio-Formats
> 
> - good example1.1sc.jpg = the jpeg version of the above file, saved
> with ImageJ
> 
> - bad example1.1sc = the original .1sc file showing an example that
> imports bad in Bio-Formats
> 
> - bad example1.1sc.jpg = the jpeg version of the above file, saved
> with ImageJ
> 
> - bad example1.jpg = the jpeg version of the .1sc file, saved by
> "Quantity One" (the BioRad software).
> 
> The imagesize is 1360 x 1024
> 
> (as a quick guide, I added the "good" version and the "bad" version
> of the "bad example" file to this E-mail in reduced format. The
> full-size images are in the zip-file).
> 
> The 2 BioRad files are taken with the same machine on the same
> computer and most of the times the conversion is perfect. Just some
> files give this error.
> 
> I get the same result when running it on a Linux system.
> 
> 
> Thanks in advance if anybody of you can shed a light on this error.
> 
> Bye,
> 
> Geert.
> 
> -- 
> Geert Meesen.
> Infrastructure Manager.
> Dep. Molecular Biotechnology, Ghent University
> Coupure Links 653
> B-9000 Gent, Belgium
> ---------------------------------------------------
> http://www.MolecularBiotechnology.UGent.be/
> E.Mail : Geert.Meesen at UGent.be
> Tel. : +32-9-264.59.68
> Fax. : +32-9-264.62.19
> E-Mail disclaimer : see http://www.ugent.be/e-maildisclaimer
> 



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