[ome-devel] FLIM

[dzmacdonald]

Hi Curtis,

That's exactly what I was looking for,

Thanks for the help,

Regards

D.


On May 1, 2010, at 12:31 AM, Curtis Rueden wrote:

> Hi Donald,
>
> Are you talking about the raw SDT files? Take a look at the Bio- 
> Formats SDT reader—there are two pieces of metadata in the original  
> metadata table, "time base" and "time bins." The "time base" is the  
> width of the histogram in nanoseconds, and "time bins" lists the  
> number of bins. So the unit of sampling for each bin (adapted from  
> the SDTReader.java code) will be:
>
> int timeBins = info.timeBins; // number of histogram bins
> int timeBase = 1e9 * info.tacR / info.tacG; // in nanoseconds
> int psPerBin = 1000 * timeBase / timeBins; // in picoseconds
>
> Or are you talking about the IMG file, or some other file format?
>
> -Curtis
>
> On Fri, Apr 30, 2010 at 10:26 AM, dzmacdonald <donald at lifesci.dundee.ac.uk 
> > wrote:
> Hi,
>
> We're working on fitting FLIM data in sdt files generated from an  
> SPC 830- Becker Hick, and calculations performed by SPCImage we're  
> working with the imported file, but looking to see where we can find  
> the unit of sampling for each bin.
> Does anyone know where we can find this? Currently we're looking in  
> the original_metadata file.
>
> Regards
>
> D.
>
>
>
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>
>

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