[ome-devel] FLIM

[Curtis Rueden]

Hi Donald,

Are you talking about the raw SDT files? Take a look at the Bio-Formats SDT
reader—there are two pieces of metadata in the original metadata table,
"time base" and "time bins." The "time base" is the width of the histogram
in nanoseconds, and "time bins" lists the number of bins. So the unit of
sampling for each bin (adapted from the SDTReader.java code) will be:

int timeBins = info.timeBins; // number of histogram bins
int timeBase = 1e9 * info.tacR / info.tacG; // in nanoseconds
int psPerBin = 1000 * timeBase / timeBins; // in picoseconds

Or are you talking about the IMG file, or some other file format?

-Curtis

On Fri, Apr 30, 2010 at 10:26 AM, dzmacdonald
<donald at lifesci.dundee.ac.uk>wrote:

> Hi,
>
> We're working on fitting FLIM data in sdt files generated from an SPC 830-
> Becker Hick, and calculations performed by SPCImage we're working with the
> imported file, but looking to see where we can find the unit of sampling for
> each bin.
> Does anyone know where we can find this? Currently we're looking in the
> original_metadata file.
>
> Regards
>
> D.
>
>
>
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>
>
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