Hi Donald,<br><br>Are you talking about the raw SDT files? Take a look at the Bio-Formats SDT reader—there are two pieces of metadata in the original metadata table, "time base" and "time bins." The "time base" is the width of the histogram in nanoseconds, and "time bins" lists the number of bins. So the unit of sampling for each bin (adapted from the SDTReader.java code) will be:<br>
<br>int timeBins = info.timeBins; // number of histogram bins<br>int timeBase = 1e9 * info.tacR / info.tacG; // in nanoseconds<br>int psPerBin = 1000 * timeBase / timeBins; // in picoseconds<br><br>Or are you talking about the IMG file, or some other file format?<br>
<br>-Curtis<br><br><div class="gmail_quote">On Fri, Apr 30, 2010 at 10:26 AM, dzmacdonald <span dir="ltr"><<a href="mailto:donald@lifesci.dundee.ac.uk">donald@lifesci.dundee.ac.uk</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin: 0pt 0pt 0pt 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;">
<div style="word-wrap: break-word;">Hi, <div><br></div><div>We're working on fitting FLIM data in sdt files generated from an<span style="font-family: 'Lucida Grande'; font-size: 11px;"> SPC 830- Becker Hick, and calculations performed by SPCImage we're working with the imported file, but looking to see where we can find the unit of sampling for each bin. </span></div>
<div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;">Does anyone know where we can find this? Currently we're looking in the original_metadata file.</span></font></div><div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;"><br>
</span></font></div><div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;">Regards</span></font></div><div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;"><br></span></font></div>
<font color="#888888"><div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;">D.</span></font></div><div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;"><br></span></font></div>
<div><font face="'Lucida Grande'" size="3"><span style="font-size: 11px;"><br></span></font></div></font></div><br>_______________________________________________<br>
ome-devel mailing list<br>
<a href="mailto:ome-devel@lists.openmicroscopy.org.uk">ome-devel@lists.openmicroscopy.org.uk</a><br>
<a href="http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-devel" target="_blank">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-devel</a><br>
<br></blockquote></div><br>