[FLIMfit-users] Bi-exponential fit

[Munro, Ian]

From: Alexandrov, Yuriy
Sent: 20 February 2018 17:42

Dear Paul,

We think you are right – this is brightness-( or “amplitude”-) weighted tau.

More specifically, it is calculated as
w_mean_tau  = sum(I0.*tau)/sum(I0)
where I0 is the pre-exponential factor, tau is the lifetime (both images certainly), the (.*) operator is pixel-wise multiplication, and summation is over all fitted pixels.

Sean may correct or comment on that (he actually implemented this calculation), - but IMO this interpretation is realistic.

Best,
Y.

On 19 Feb 2018, at 15:10, Paul Thomas (SCI - Staff) via FLIMfit-users <flimfit-users at lists.openmicroscopy.org.uk<mailto:flimfit-users at lists.openmicroscopy.org.uk>> wrote:

Hello,

I’m using FLIMfit to fit bi-exponential decay curves to TCSPC data in FRET experiments. I’ve seen discussions that suggest using the “amplitude-weighted” average tau for such experiments. Is this the value designated “w_mean_tau” in the Parameters list of FLIMfit?

Thanks,
Paul.
__________________________________________
Dr. Paul Thomas FRMS FPhysiol, Manager,
The Henry Wellcome Laboratory for Cell Imaging,
Faculty of Science,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.
e-mail: p.thomas at uea.ac.uk<mailto:p.thomas at uea.ac.uk>
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people



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