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<b class=""><span lang="EN-US" class="">From:</span></b><span lang="EN-US" class=""> Alexandrov, Yuriy <br class="">
<b class="">Sent:</b> 20 February 2018 17:42<br class="">
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<span style="color: rgb(31, 73, 125);" class="">Dear Paul,<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""> </span></div>
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<span style="color: rgb(31, 73, 125);" class="">We think you are right – this is brightness-( or “amplitude”-) weighted tau.<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""> </span></div>
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<span style="color: rgb(31, 73, 125);" class="">More specifically, it is calculated as<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class="">w_mean_tau</span><span style="color: rgb(31, 73, 125);" class=""> = sum(I0.*tau)/sum(I0)<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class="">where I0 is the pre-exponential factor, tau is the lifetime (both images certainly), the (.*) operator is pixel-wise multiplication, and summation is over all fitted pixels.<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""> </span></div>
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<span style="color: rgb(31, 73, 125);" class="">Sean may correct or comment on that (he actually implemented this calculation), - but IMO this interpretation is realistic.<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""> </span></div>
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<span style="color: rgb(31, 73, 125);" class="">Best,<o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class="">Y. <o:p class=""></o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""> </span></p>
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<div class="">On 19 Feb 2018, at 15:10, Paul Thomas (SCI - Staff) via FLIMfit-users <<a href="mailto:flimfit-users@lists.openmicroscopy.org.uk" class="">flimfit-users@lists.openmicroscopy.org.uk</a>> wrote:</div>
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<span style="font-family: 'Book Antiqua', serif;" class="">Hello,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class="">I’m using FLIMfit to fit bi-exponential decay curves to TCSPC data in FRET experiments. I’ve seen discussions that suggest using the “amplitude-weighted” average tau for such experiments. Is this the
value designated “w_mean_tau” in the Parameters list of FLIMfit?<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class="">Thanks,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif;" class="">Paul.<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">__________________________________________<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Dr. Paul Thomas FRMS FPhysiol, Manager,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">The Henry Wellcome Laboratory for Cell Imaging,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Faculty of Science,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">University of East Anglia,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Norwich Research Park,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Norwich,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">NR4 7TJ,<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">United Kingdom.<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">e-mail:<span class="Apple-converted-space"> </span><a href="mailto:p.thomas@uea.ac.uk" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span style="color: blue;" class="">p.thomas@uea.ac.uk</span></a><o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Tel: +44-1603-592196<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Fax: +44-1603-592250<o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Imaging web-site:<span class="Apple-converted-space"> </span><a href="https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span style="color: blue;" class="">https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab</span></a><o:p class=""></o:p></span></div>
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<span style="font-family: 'Book Antiqua', serif; color: rgb(68, 84, 106);" class="">Personal web-page:<span class="Apple-converted-space"> </span><a href="https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span style="color: blue;" class="">https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people</span></a></span><span style="font-family: 'Book Antiqua', serif;" class=""><o:p class=""></o:p></span></div>
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<span style="font-size: 9pt; font-family: Arial, sans-serif;" class=""><o:p class=""> </o:p></span></div>
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<span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
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</span><span style="font-family: Arial, sans-serif;" class=""><span id="cid:image001.png@01D3A993.D139D030"><image001.png></span></span><span style="" class=""><o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></b></div>
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<b class=""><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">Gold (</span></b><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">Teaching Excellence Framework 2017)<o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">UK Top 15<span class="Apple-converted-space"> </span></span></b><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">(The
Times/Sunday Times 2018 and Complete University Guide 2018)<o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">World Top 200<span class="Apple-converted-space"> </span></span></b><span style="font-size: 9pt; font-family: Arial, sans-serif; color: rgb(31, 73, 125);" class="">(Times
Higher Education World University Rankings 2017)<b class=""><o:p class=""></o:p></b></span></div>
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<o:p class=""> </o:p></div>
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