[FLIMfit-users] Bi-exponential fit

Paul Thomas (SCI - Staff) P.Thomas at uea.ac.uk
Mon Feb 19 15:10:48 GMT 2018


Hello,

I'm using FLIMfit to fit bi-exponential decay curves to TCSPC data in FRET experiments. I've seen discussions that suggest using the "amplitude-weighted" average tau for such experiments. Is this the value designated "w_mean_tau" in the Parameters list of FLIMfit?

Thanks,
Paul.
__________________________________________
Dr. Paul Thomas FRMS FPhysiol, Manager,
The Henry Wellcome Laboratory for Cell Imaging,
Faculty of Science,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.
e-mail: p.thomas at uea.ac.uk<mailto:p.thomas at uea.ac.uk>
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people



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