[FLIMfit-users] FLIMfit help

[Munro, Ian]

Hi Jorge

You can load an irf from any image using IRF->Load IRF.

You can also generate an irf from an area of an image
as described in

http://lists.openmicroscopy.org.uk/pipermail/ome-users/2014-September/004769.html

If nothing else this will generate an example of a .csv file that FLIMfit can handle

All the best.

Ian



On 18 Sep 2014, at 23:06, Jorge Palma Chavez <jorgepalma89 at tamu.edu<mailto:jorgepalma89 at tamu.edu>> wrote:

Hi Ian,

I am wondering if you could help to load the IRF to FLIMfit, I tried writing a ‘.csv’ file from the IRF array (1x190), but I am getting error messages . I really appreciate your help.

Best Regards,
Jorge

On Fri, Aug 29, 2014 at 11:45 AM, Munro, Ian <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:
Hi Jorge

That’s excellent news! Nice work & thanks for letting me know!

Ian



On 29 Aug 2014, at 17:38, Jorge Palma Chavez <jorgepalma89 at tamu.edu<mailto:jorgepalma89 at tamu.edu>> wrote:

Ian,

Thanks for your advice, I finally imported my files to FLIMfit. I will be working on it.

Best Regards,

Jorge Palma


On Fri, Aug 22, 2014 at 10:36 AM, Munro, Ian <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:
Ok cool. If you run into problems just let me know.
FLIMfit 4.7.0 & bio-formats 5.0.3 have now been released so that should simplify things a bit.

Ian


On 22 Aug 2014, at 16:34, Jorge Palma Chavez <jorgepalma89 at tamu.edu<mailto:jorgepalma89 at tamu.edu>> wrote:


Ian,

No, I have to keep working on it. I had been busy with other research project but we just finished. I will let you know later.

Thanks,

Jorge

El ago 22, 2014 10:23 a.m., "Munro, Ian" <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> escribió:
Hi Jorge.

Did you manage to write your files as ome-tiffs?

Ian


On 22 Aug 2014, at 16:16, Jorge Palma Chavez <jorgepalma89 at tamu.edu<mailto:jorgepalma89 at tamu.edu>> wrote:

Hi Ian,

Unfortunately, I have not been able to import my data to FLIMfit yet. I am receiving the same error message over and over. Were you able to import my files to FLIMfit the last time? I am really interested to use you software but  it has been a little confusing for me.

Regards,

Jorge Palma


On Fri, Aug 22, 2014 at 8:04 AM, Munro, Ian <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:
Sorry Jorge

Please ignore the last mail.
Sent by accident

Ian


On 22 Aug 2014, at 14:03, Ian Munro <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:

> & the other side
> https://github.com/openmicroscopy/Imperial-FLIMfit/blob/master/FLIMfitFrontEnd/Classes/%40flim_data_series/get_image_dimensions.m
>
> line 171 -ish.
>
> Perhaps he has  an old FLIMfit?
>
> Ian
>
>
> On 22 Aug 2014, at 13:49, Ian Munro <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:
>
>> Hi again Jorge
>>
>> Just wanted to check how you’re doing. You’ve been so quiet that it’s either going very well
>> or very poorly.
>>
>> All the best
>>
>> Ian
>>
>>
>> On 30 Jul 2014, at 19:14, Ian Munro <i.munro at imperial.ac.uk<mailto:i.munro at imperial.ac.uk>> wrote:
>>
>>> Hi again Jorge
>>>
>>>
>>> As I’ve pointed you to a lot of open microscopy environment (OME) stuff it’s probably worth being aware of the forums at
>>>
>>> https://www.openmicroscopy.org/community/
>>>
>>> Currently our work on FLIMfit is being funded by the OME project.
>>> As what you are trying ( the first group outside Imperial College to use FLIMfit for
>>> Gated , as opposed to TCSPC, FLIM) is so interesting
>>> it would be helpful to have a record that anyone trying the same thing  could access in the future.
>>>
>>> It’s also a good resource for questions on , for example, bio-fomats.
>>>
>>> Regards
>>>
>>> Ian
>>>
>>>
>>>
>>>
>>
>








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