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<div>Hi Jorge</div>
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<div>You can load an irf from any image using IRF->Load IRF. </div>
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<div>You can also generate an irf from an area of an image </div>
<div>as described in </div>
<div><br>
</div>
<div><a href="http://lists.openmicroscopy.org.uk/pipermail/ome-users/2014-September/004769.html">http://lists.openmicroscopy.org.uk/pipermail/ome-users/2014-September/004769.html</a></div>
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<div>If nothing else this will generate an example of a .csv file that FLIMfit can handle</div>
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<div>All the best.</div>
<div><br>
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<div>Ian</div>
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<div>On 18 Sep 2014, at 23:06, Jorge Palma Chavez <<a href="mailto:jorgepalma89@tamu.edu">jorgepalma89@tamu.edu</a>> wrote:</div>
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<div>Hi Ian,</div>
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</div>
<div>I am wondering if you could help to load the IRF to FLIMfit, I tried writing a ‘.csv’ file from the IRF array (1x190), but I am getting error messages . I really appreciate your help.</div>
<div><br>
</div>
<div>Best Regards,</div>
<div>Jorge</div>
</div>
<div class="gmail_extra"><br>
<div class="gmail_quote">On Fri, Aug 29, 2014 at 11:45 AM, Munro, Ian <span dir="ltr">
<<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>></span> wrote:<br>
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<div>Hi Jorge</div>
<div><br>
</div>
That’s excellent news! Nice work & thanks for letting me know! <span class="HOEnZb">
<font color="#888888">
<div><br>
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<div>Ian </div>
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<div>On 29 Aug 2014, at 17:38, Jorge Palma Chavez <<a href="mailto:jorgepalma89@tamu.edu" target="_blank">jorgepalma89@tamu.edu</a>> wrote:</div>
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<div>Ian,</div>
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<div>Thanks for your advice, I finally imported my files to FLIMfit. I will be working on it.</div>
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<div>Best Regards,</div>
<div><br>
</div>
<div>Jorge Palma</div>
</div>
<div class="gmail_extra"><br>
<br>
<div class="gmail_quote">On Fri, Aug 22, 2014 at 10:36 AM, Munro, Ian <span dir="ltr">
<<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div style="word-wrap:break-word">Ok cool. If you run into problems just let me know.
<div>FLIMfit 4.7.0 & bio-formats 5.0.3 have now been released so that should simplify things a bit.<span><font color="#888888"><br>
<div><br>
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<div>Ian</div>
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<div>On 22 Aug 2014, at 16:34, Jorge Palma Chavez <<a href="mailto:jorgepalma89@tamu.edu" target="_blank">jorgepalma89@tamu.edu</a>> wrote:</div>
<br>
<blockquote type="cite">
<p dir="ltr">Ian,</p>
<p dir="ltr">No, I have to keep working on it. I had been busy with other research project but we just finished. I will let you know later.</p>
<p dir="ltr">Thanks,</p>
<p dir="ltr">Jorge</p>
<div class="gmail_quote">El ago 22, 2014 10:23 a.m., "Munro, Ian" <<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>> escribió:<br type="attribution">
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<div style="word-wrap:break-word">Hi Jorge.
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</div>
<div>Did you manage to write your files as ome-tiffs?</div>
<div><br>
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<div>Ian</div>
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<div>On 22 Aug 2014, at 16:16, Jorge Palma Chavez <<a href="mailto:jorgepalma89@tamu.edu" target="_blank">jorgepalma89@tamu.edu</a>> wrote:</div>
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<div>Hi Ian,</div>
<div><br>
</div>
<div>Unfortunately, I have not been able to import my data to FLIMfit yet. I am receiving the same error message over and over. Were you able to import my files to FLIMfit the last time? I am really interested to use you software but it has been a little confusing
for me.<br>
</div>
<div><br>
</div>
<div>Regards,</div>
<div><br>
</div>
<div>Jorge Palma</div>
</div>
<div class="gmail_extra"><br>
<br>
<div class="gmail_quote">On Fri, Aug 22, 2014 at 8:04 AM, Munro, Ian <span dir="ltr">
<<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
Sorry Jorge<br>
<br>
Please ignore the last mail.<br>
Sent by accident<br>
<span><font color="#888888"><br>
Ian<br>
</font></span>
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<div><br>
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On 22 Aug 2014, at 14:03, Ian Munro <<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>> wrote:<br>
<br>
> & the other side<br>
> <a href="https://github.com/openmicroscopy/Imperial-FLIMfit/blob/master/FLIMfitFrontEnd/Classes/%40flim_data_series/get_image_dimensions.m" target="_blank">
https://github.com/openmicroscopy/Imperial-FLIMfit/blob/master/FLIMfitFrontEnd/Classes/%40flim_data_series/get_image_dimensions.m</a><br>
><br>
> line 171 -ish.<br>
><br>
> Perhaps he has an old FLIMfit?<br>
><br>
> Ian<br>
><br>
><br>
> On 22 Aug 2014, at 13:49, Ian Munro <<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>> wrote:<br>
><br>
>> Hi again Jorge<br>
>><br>
>> Just wanted to check how you’re doing. You’ve been so quiet that it’s either going very well<br>
>> or very poorly.<br>
>><br>
>> All the best<br>
>><br>
>> Ian<br>
>><br>
>><br>
>> On 30 Jul 2014, at 19:14, Ian Munro <<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>> wrote:<br>
>><br>
>>> Hi again Jorge<br>
>>><br>
>>><br>
>>> As I’ve pointed you to a lot of open microscopy environment (OME) stuff it’s probably worth being aware of the forums at<br>
>>><br>
>>> <a href="https://www.openmicroscopy.org/community/" target="_blank">https://www.openmicroscopy.org/community/</a><br>
>>><br>
>>> Currently our work on FLIMfit is being funded by the OME project.<br>
>>> As what you are trying ( the first group outside Imperial College to use FLIMfit for<br>
>>> Gated , as opposed to TCSPC, FLIM) is so interesting<br>
>>> it would be helpful to have a record that anyone trying the same thing could access in the future.<br>
>>><br>
>>> It’s also a good resource for questions on , for example, bio-fomats.<br>
>>><br>
>>> Regards<br>
>>><br>
>>> Ian<br>
>>><br>
>>><br>
>>><br>
>>><br>
>><br>
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