[ome-users] missing images reading Nikon nd2 files

Curtis Rueden ctrueden at wisc.edu
Mon Jul 14 22:06:08 BST 2014


Hi everyone,

> Importing nd2 files (two) delivers one (3-channel) image less than the
> NIS Elements Viewer.

For the archives: this report emerged from a related thread on the ImageJ
mailing list, which you can find at:
https://list.nih.gov/cgi-bin/wa.exe?A2=IMAGEJ;cc9bcbc2.1407

Regards,
Curtis


On Mon, Jul 14, 2014 at 3:25 PM, Karsten <karo13de at googlemail.com> wrote:

>
> Importing nd2 files (two) delivers one (3-channel) image less than the NIS
> Elements Viewer.
>
> This happens under ImageJ, Fiji and the commandline tool showinf.
>
> The meta data show the reduced number of images, still the list of the
> scanning table coordinates show the full number of images extended by a
> constant dummy coordinate to the number of (1-channel) images found from
> bioformats software.
>
> I attache the output of showinf.
>
> The second file delivers also one image less than the Nikon Viewer.
> Additionally the metadata show a different structure by dividing the list
> of images in a large number of series. According the microscope users, they
> did not change anything saving the second data file.
>
> One image less is possibly not so important. However for the sake of
> correctness it could be improved.
>
> Thanks in advance for any help
>
> Karsten
> karo13de at googlemail.com
>
>
>
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>
>
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