[ome-users] nd2 files opening with multiple series instead of time series

Cammer, Michael Michael.Cammer at nyulangone.org
Wed Sep 12 22:52:20 BST 2018


Last week we used a Nikon W1 spinning disk system.  2 channel Z series were taken at multiple positions through time.  Each XY location is a separate ND2 file.

When we open them with BioFormats 5.9.2 with jave 1.8, we are prompted for Series whereas they should be a single file of timepoints.  It's easy to combine them with the macro below, but would be great to have them open as a single time series.  Happy to upload an example file if you would like.

Also, the title is doubled.

Thank you!


Best regards,
Michael Cammer

The macro to fix the problem afterwards:

    t = getTitle;
    run("Concatenate...", "all_open title=[nmg smc_xy1] open");
    run("Stack to Hyperstack...", "order=xyczt(default) channels=2 slices=31 frames=37 display=Composite");
    rename(t);
    // manually edit title on save

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