[ome-users] nd2 files from Nikon DS-Fi3 camera are not read correctly

MEYENHOFER Felix felix.meyenhofer at unifr.ch
Tue Oct 2 09:01:55 BST 2018


Hi David

thanks for looking into this. 

Best

Felix

> On 27 Sep 2018, at 16:01, David Gault (Staff) <d.gault at dundee.ac.uk> wrote:
> 
> Hi Felix,
> 
> Thank you for reporting this problem and providing a sample file. I have been able to test and reproduce the issue as you described and it does indeed appear to be a bug.
> I have opened a Trello card on the Bio-Formats board with the details and some of the initial investigation:
> https://trello.com/c/EtOwZ7MH/283-nd2-incorrect-channels-and-pixel-data
> 
> With Thanks,
> David Gault
> 
>> On 26 Sep 2018, at 09:28, MEYENHOFER Felix <felix.meyenhofer at unifr.ch> wrote:
>> 
>> Dear OMERO Team
>> 
>> The following image causes problems when I try to open it using Fiji and Bio-Formats. Bellow is the image shown with the NIS Elements Viewer.
>> 
>> <Screen Shot 2018-09-26 at 10.14.16.png>
>> 
>> 
>> The window that is produced by Fiji shows only a part of the original image.
>> <Screen Shot 2018-09-26 at 10.17.13.png>
>> 
>> 
>> A closer look, shows that the C and X-Y dimensions seem to be messed up. 
>> <Screen Shot 2018-09-26 at 10.17.34.png>
>> 
>> Finally the parsing of the metadata seems to have trouble too, given that the colors are not coherent with the image acquisition and given that the scale factor too seems to be missing in the displayed image in Fiji.
>> The original file can be found in the following link:
>> https://drive.switch.ch/index.php/s/QsroyTOOq6cDQXy
>> 
>> Is there a work-around, or could you confirm this being a bug?
>> 
>> Best regards, 
>> 
>> Felix 
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> 
> 
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