[ome-users] Issue opening Z-stack ND2 file

[David Gault (Staff)]

Hi Thomas,

The image should by default open as a hyper stack image containing the multiple channels and Z slices with multiple sliders to control each.
The display of these can be controlled as in the link in the previous reply or the channels can additionally be combined or separated via the Bio-Formats import options.

If you are only seeing a single slider to control the channels but not for the Z slices then would it possible to upload the file you are using to the below link for testing?
https://www.openmicroscopy.org/qa2/qa/upload/

With Thanks,
David Gault

On 10 Jan 2017, at 13:31, Cammer, Michael <Michael.Cammer at med.nyu.edu<mailto:Michael.Cammer at med.nyu.edu>> wrote:

http://microscopynotes.com/imagej/hyperstackimages01/index.html




_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopy
http://microscopynotes.com/
Cell: (914) 309-3270
________________________________
From: ome-users [ome-users-bounces at lists.openmicroscopy.org.uk<mailto:ome-users-bounces at lists.openmicroscopy.org.uk>] on behalf of wa lao [hongwab at gmail.com<mailto:hongwab at gmail.com>]
Sent: Monday, January 09, 2017 7:02 PM
To: ome-users at lists.openmicroscopy.org.uk<mailto:ome-users at lists.openmicroscopy.org.uk>
Subject: [ome-users] Issue opening Z-stack ND2 file

Dear Sir/Madam,
This is a Fiji user who was trying to open a z-stack ND2 file (Z=5) with 3 channels. However, it only pop up as a single image with a slide bar which I can switch between channels. Normally it will show stack of images automatically as well, am I right? It looks fine when I open it with NIS elements Ar.

I posted this issue on the forum and a nice guy responded and suggested I should contact the develops. Wish I am not sending this email to a wrong place. (http://forum.imagej.net/t/cannot-open-nd2-z-stack/3676<https://urldefense.proofpoint.com/v2/url?u=http-3A__forum.imagej.net_t_cannot-2Dopen-2Dnd2-2Dz-2Dstack_3676&d=DQMFAg&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=rysnZd_UOwdMe_w6IINVDav0Cyl1itNoahw_aqdiGvE&s=Bv_S_8bpFLpdheFLa9TDhqQOVt8-Le9Kf0WkUrFxZeA&e=>)

I am using Mac with the latest version of Fiji. Could you give me some suggestions on fixing this issue please?

Best Regards,
Thomas Lao

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