[ome-users] Problem importing stiched images

Kai Schleicher kai.schleicher at unibas.ch
Wed Apr 27 20:05:26 BST 2016


Hi,

I acquired tiled images on a FEI MORE widefield system (recorded each tile as an individual .tf8) that where then deconvolved using Huygens remote manger (saved as ics/ids) and subsequently stitched in FIJI using the grid/collection stitching plugin (http://imagej.net/Grid/Collection_Stitching_Plugin). The final stitched image as well as a maximum projection was saved both as ics/ids and ome.tiff via the bio-formats and then imported in the latest OMERO version (your demo server, dataset ID 5468).

While there were no errors during import, the images can't be viewed in OMERO but instead are displayed as gray images. Do you have an idea what might have happened? The stitched images are displayed perfectly fine in FIJI using the latest bio-formats.

Thanks and cheers,
Kai

--
Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
Phone: +41 61 267 22 50 | kai.schleicher at unibas.ch | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.openmicroscopy.org.uk/pipermail/ome-users/attachments/20160427/48d8e31b/attachment.html>


More information about the ome-users mailing list