[ome-users] photoconversion of td-Tomato in diaminobenzidine

[Roger Leigh]

On 20/03/15 13:06, Marshak, David W wrote:
> Does anyone know of a reliable method for photoconverting td-Tomato to an osmiophilic substance for electron microscopy?

Dear David,

This is probably not the most appropriate list for this type of
question, since this list is for OME software-related questions and
discussion, rather than staining techniques.

One (possibly naïve) suggestion would be to insert a tetracysteine motif
into your td-Tomato and then use ReAsH-catalysed DAB photoconversion.
Unless the td-Tomato protein causes any complications; I've not tried
this personally.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021176/


Regards,
Roger

--
Dr Roger Leigh -- Open Microscopy Environment
Wellcome Trust Centre for Gene Regulation and Expression,
College of Life Sciences, University of Dundee, Dow Street,
Dundee DD1 5EH Scotland UK   Tel: (01382) 386364

The University of Dundee is a registered Scottish Charity, No: SC015096