[ome-users] CZI dimension

[Paul Thomas (SCI)]

I tried this, but it didn't seem to make much difference to the fit.
Paul.

____________________
Dr. Paul Thomas, Manager,
The Henry Wellcome Laboratory for Cell Imaging,
Faculty of Science,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.

e-mail: p.thomas at uea.ac.uk
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people


> -----Original Message-----
> From: Munro, Ian [mailto:i.munro at imperial.ac.uk]
> Sent: 07 March 2014 11:56
> To: Paul Thomas (SCI)
> Subject: Re: [ome-users] CZI dimension
> 
> Hi Paul
> 
> That's good news.
> One thing to be aware of is that FLIMfit, by default, allows  for
> residual fluorescence from the previous excitation pulse.
> 
> I'm afraid I know nothing about the ImSpector software but perhaps
> turning this option off in FLIMfit might shed some light (Ow!)
> 
> The option is in "Advanced" "Pulse train Correction'.
> 
> Regards
> 
> Ian
> 
> On 7 Mar 2014, at 11:46, Paul Thomas (SCI) <P.Thomas at uea.ac.uk> wrote:
> 
> > Hi Ian,
> >
> > FYI: I was able to reproduce the fit of ImSpector using FLIMfit by
> using the Maximum Likelihood algorithm and adjusting the background. I
> initially tried using a background that was equal to the "offset"
> calculated by ImSpector, but found I had to increase the offset value
> ~2x to get good agreement between the two programs.
> >
> > Thanks,
> > Paul.
> > ____________________
> > Dr. Paul Thomas, Manager,
> > The Henry Wellcome Laboratory for Cell Imaging, Faculty of Science,
> > University of East Anglia, Norwich Research Park, Norwich,
> > NR4 7TJ,
> > United Kingdom.
> >
> > e-mail: p.thomas at uea.ac.uk
> > Tel: +44-1603-592196
> > Fax: +44-1603-592250
> > Imaging web-site:
> > https://www.uea.ac.uk/biological-sciences/research/facilities/henry-
> we
> > llcome-lab Personal web-page:
> > https://www.uea.ac.uk/biological-sciences/research/facilities/henry-
> we
> > llcome-lab/people
> >
> >> -----Original Message-----
> >> From: Munro, Ian [mailto:i.munro at imperial.ac.uk]
> >> Sent: 06 March 2014 18:28
> >> To: Paul Thomas (SCI)
> >> Subject: Re: [ome-users] CZI dimension
> >>
> >>
> >> Dear Paul
> >>
> >> Please also note our discussion on
> >> https://www.openmicroscopy.org/community/viewtopic.php?f=4&t=7429
> >> where I cover some of the same ground.
> >>
> >> Regards
> >>
> >> ian