[ome-users] CZI dimension

[Munro, Ian]

> Hi Paul
> 
> That’s good news.
> One thing to be aware of is that FLIMfit, by default, allows  for residual fluorescence from the previous excitation pulse.
> 
> I’m afraid I know nothing about the ImSpector software but perhaps turning this option off in FLIMfit
> might shed some light (Ow!) 
> 
> The option is in “Advanced” “Pulse train Correction’.
> 
> Regards
> 
> Ian
> 
> On 7 Mar 2014, at 11:46, Paul Thomas (SCI) <P.Thomas at uea.ac.uk> wrote:
> 
>> Hi Ian,
>> 
>> FYI: I was able to reproduce the fit of ImSpector using FLIMfit by using the Maximum Likelihood algorithm and adjusting the background. I initially tried using a background that was equal to the "offset" calculated by ImSpector, but found I had to increase the offset value ~2x to get good agreement between the two programs.
>> 
>> Thanks,
>> Paul.
>> ____________________
>> Dr. Paul Thomas, Manager,
>> The Henry Wellcome Laboratory for Cell Imaging,
>> Faculty of Science,
>> University of East Anglia,
>> Norwich Research Park,
>> Norwich,
>> NR4 7TJ,
>> United Kingdom.
>> 
>> e-mail: p.thomas at uea.ac.uk
>> Tel: +44-1603-592196
>> Fax: +44-1603-592250
>> Imaging web-site: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
>> Personal web-page: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people
>> 
>>> -----Original Message-----
>>> From: Munro, Ian [mailto:i.munro at imperial.ac.uk]
>>> Sent: 06 March 2014 18:28
>>> To: Paul Thomas (SCI)
>>> Subject: Re: [ome-users] CZI dimension
>>> 
>>> 
>>> Dear Paul
>>> 
>>> Please also note our discussion on
>>> https://www.openmicroscopy.org/community/viewtopic.php?f=4&t=7429
>>> where I cover some of the same ground.
>>> 
>>> Regards
>>> 
>>> ian
>