[ome-users] Imaris .ims wrong number of channels

[Roger Leigh]

On 01/04/2014 13:12, Maier, Lorenz wrote:
> Dear all,
>
> there seems to be an error in the number of channels detected for Imaris
> files (.ims 5.5 format, generated with Imaris 7.7).
>
> Fiji opens shows only one of the channels. I’ve uploaded an example on
> https://www.openmicroscopy.org/qa2/qa/feedback/8019 .

Hi,

In case you didn't get the reply from the QA system for #8022 (CZI
channel colour), following up here as well.

I've checked this CZI image with the current 5.0.x build of the
loci_tools plugin and ImageJ 1.48s.  It's displaying
green/blue/red/white correctly as far as I can tell. I imported your
image into ImageJ using the Bio-Formats importer with either "Composite"
or "Colourised" channel mode selected and the channels all appear as the
correct colour. (The channel mode shouldn't affect the import itself
though, just the default view mode.)

Which version of the Bio-Formats plugin were you using?

Depending upon which version of the plugin you are using, it's possible
that this might be fixed in the upcoming 5.0.1 release next week, but if
you're using a 4.4 version of the plugin, it would be worth checking
with 5.0.0.

Regards,
Roger

--
Dr Roger Leigh -- Open Microscopy Environment
Wellcome Trust Centre for Gene Regulation and Expression,
College of Life Sciences, University of Dundee, Dow Street,
Dundee DD1 5EH Scotland UK   Tel: (01382) 386364

The University of Dundee is a registered Scottish Charity, No: SC015096