[ome-users] Leica lif file problem

Wood, Christopher CJW at stowers.org
Wed Nov 6 15:28:38 GMT 2013


Hi,

I am uploading a Leica lif file through the OME qa upload page. This file
contains 21 3-channel images that in both Leica software and Imaris show
the maximum pixel value as 4095. Opening in Fiji and Omero, the first
channel seems to have values up to 2^16. In Fiji, the histogram of the
first channel looks like noise from a uniform distribution, and the
histogram of the third channel does not match what I see in other software
either.

Thanks

Chris




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