[ome-users] Leica .lif import of stacks from tile scanning

[Kai Toellner]

I am importing stacks of four channel (4C) tile scans from a Leica microscope, written as .lif files, into Fiji. Fiji is automatically updating and just updated Loci bioformats to vers. 4.4.9-DEV rev 34f3393, built 7 May 13. Imagej is vers. 1.47q. The problem I describe is reproducible under Windows and iOS.
Before the update different stacks of 4C tile scans were each showing as individual images (series) on the second page of the bio-formats plugin (Choose series). To import the whole stack I had to only select one series.
Since the most recent update all tiles of the tile scan show as individual series, meaning to import the whole stack I have to mark and import all individual images, which results in the import of dozens of individual images, not one single stack.
This upsets a macro I have just written that relies on the import of a stack of tiles, not many separate tiles.
Question: Is this a bug of the latest version of bioformats and will be fixed at some stage, or is this a new feature that will stay permanently?

Kai

Dr. Kai-Michael Toellner
Senior Research Fellow
School of Immunity and Infection
Institute for Biomedical Research (IBR) room 432
Medical School
University of Birmingham
Birmingham B15 2TT
United Kingdom
Tel: +44 121 41 58687
FAX: +44 121 41 43599

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