[ome-users] Can I convert 1-bit PNG files to 1-bit OME.TIFF files?

[Ghislain Bonamy]

Frans,

 

Actually what would be even more powerful would be to be able to embed
the bitmaps as ROIs. I believe ROI (polygons) are supported by
OME-Tiff/OME-XML as an SVG map. The question is how can bitmaps be
converted as such. I am not sure what format imageJ uses to male its ROI
using the particle detector. Perhaps someone has an easy way to do this?

 

Best,

 

Ghislain Bonamy, PhD

__________________________________________

Research Investigator I

 

Genomic Institute of the

Novartis Research

Foundation

Department of Molecular & Cell Biology, room G214

10675 John Jay Hopkins Drive

San Diego CA 92121

USA

 

+1 (858) 812-1534 (W & F)

+1 (757) 941-4194 (H)

+1 (858) 354-7388 (M)

www.gnf.org

 

Hudson-Alpha Institute for Biotechnology

www.hudsonalpha.org

 

From: ome-users-bounces at lists.openmicroscopy.org.uk
[mailto:ome-users-bounces at lists.openmicroscopy.org.uk] On Behalf Of
Cornelissen, Frans [PRDBE]
Sent: Monday, January 05, 2009 6:50 AM
To: ome-users at lists.openmicroscopy.org.uk
Subject: [ome-users] Can I convert 1-bit PNG files to 1-bit OME.TIFF
files?

 

All, 

best wishes for 2009

Is it possible to convert 1-bit PNG files (cell-boundaries as a binay
image) to 1-bit OME.Tiff  files?

If I use the bfconvert command line tool, it ends up as a UINT8 image...

The (compressed) PNG file is only 4 K, the Tiff becomes 3900 K.

I have individual  overlay files for the CellBodies, the Nuclei and the
Neurites.

Ultimately, I want to import these PNG files as new "Channels" of an
existing file (with already 2 channels) to show it as an "overlay" in
the Insight Viewer.

Are there easier ways to do this ?

 

Thanks,

Best regads, frans

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