[lm-announce] What to do when the DeltaVision "looks funny"

[Sam Swift]

Dear All,

We have compiled a list of the most common fixes on the DeltaVisions  
when "the fluorescence is weak" or "I can only see part of the field  
of view" or "the green and the red should be in the same place and  
they're not".  This is a list of simple things you can check yourself  
but, as ever, please come and ask us if you want any help  
whatsoever.  If any of you have an interest in what these bits  
actually do, please pop down for a chat.  We even have a coffee machine.

Could I also again remind people doing DIC and experiments with the  
QLM to put the microscope back to the "standard configuration" after  
use i.e. prism and analyser out, laser mirror out.

Cheers!
Sam


If you are NOT doing Kohler, DIC or a Photokintetics experiment, and  
you have problems, check the following:

Clean the lens (an oil bubble will cause weak fluorescence or an  
obstructed field of view)
Fluorescence Shutter under objective lens needs to be OPEN (otherwise  
you won't see any fluorescence)
Dichroic mirror in correct position and locked in place (will cause a  
weak signal, no signal, or an obstructed field of view)
DIC prism and analyser should be OUT (or you'll lose lots of trans or  
fluorescence light, plus have all kinds of weird stuff going on in  
fluorescence)
Laser mirror should be in OUT position (or you'll cut some light out  
in fluorescence)
UV blocking filter should be in OUT position (or you won't see DAPI  
or much CFP)
Transmitted light aperture needs to be OPEN (if the condenser is out  
of alignment and this is closed, you won't see any transmitted light)
The eyepiece lens should be set to 0 (ie the Bertrand lens – CT  
position – should not be in place, otherwise you'll be looking at the  
back of the lens, not the top where your sample is)
Aux Mag should be OUT (i.e. a 1x mag NOT 1.5x mag, you'll lose light  
and your sample will look very big, the stage won't move properly etc)
If you still have a problem with “alignment” or “some sort of circle  
eclipsing your image” check the lens, eyepiece filter and photoframe  
are properly seated (is the lens locked in place, is the photoframe  
halfway out, is the eyepiece filter locked in place?)
If you have done Kohler, DIC or a Photokinetics experiment, you MUST  
leave the system in the above configuration when you have finished.


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