<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear All,
<div><br class="webkit-block-placeholder"></div><div>We have compiled a list of the most common fixes on the DeltaVisions when "the fluorescence is weak" or "I can only see part of the field of view" or "the green and the red should be in the same place and they're not". This is a list of simple things you can check yourself but, as ever, please come and ask us if you want any help whatsoever. If any of you have an interest in what these bits actually do, please pop down for a chat. We even have a coffee machine.</div><div><br class="webkit-block-placeholder"></div><div>Could I also <i>again</i> remind people doing DIC and experiments with the QLM to put the microscope back to the "standard configuration" after use i.e. prism and analyser out, laser mirror out.</div><div><br></div><div>Cheers!</div><div>Sam</div><div><br class="webkit-block-placeholder"></div><div><br class="webkit-block-placeholder"></div><!--StartFragment--> <div class="MsoNormal"><b>If you are NOT doing Kohler, DIC or a Photokintetics experiment, and you have problems, check the following: </b></div><p class="MsoNormal" style="margin-left:18.0pt"><b><o:p></o:p></b></p> <ol style="margin-top:0cm" start="1" type="1"> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Clean the lens (an oil bubble will cause weak fluorescence or an obstructed field of view)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Fluorescence Shutter under objective lens needs to be OPEN (otherwise you won't see any fluorescence)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Dichroic mirror in correct position and locked in place (will cause a weak signal, no signal, or an obstructed field of view)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>DIC prism and analyser should be OUT (or you'll lose lots of trans or fluorescence light, plus have all kinds of weird stuff going on in fluorescence)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Laser mirror should be in OUT position (or you'll cut some light out in fluorescence)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>UV blocking filter should be in OUT position (or you won't see DAPI or much CFP)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Transmitted light aperture needs to be OPEN (if the condenser is out of alignment and this is closed, you won't see any transmitted light)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>The eyepiece lens should be set to 0 (ie the Bertrand lens – CT position – should not be in place, otherwise you'll be looking at the back of the lens, not the top where your sample is)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>Aux Mag should be OUT (i.e. a 1x mag NOT 1.5x mag, you'll lose light and your sample will look very big, the stage won't move properly etc)</b></li> <li class="MsoNormal" style="mso-list:l0 level1 lfo1;tab-stops:list 36.0pt"><b>If you still have a problem with “alignment” or “some sort of circle eclipsing your image” check the lens, eyepiece filter and photoframe are properly seated (is the lens locked in place, is the photoframe halfway out, is the eyepiece filter locked in place?)</b></li></ol> <div class="MsoNormal"><b>If you have done Kohler, DIC or a Photokinetics experiment, you MUST leave the system in the above configuration when you have finished.</b></div> <!--EndFragment--> <div><b> </b></div><div><b><br class="webkit-block-placeholder"></b></div></body></html>