[lm-announce] 2nd floor spectris: revised filters

[Sam Swift]

Dear All,

We have revised the filter configuration on the 2 floor spectris as  
follows:

EX/EM:  DAPI  FITC  CFP  YFP  GFP mCherry
DCs:  QUAD, JP4, JP3, GFP/mCherry

This system has 3 laser lines for photokinetic experiments (406, 488,  
532).  Although the FRET dichroic has been replaced, it is still  
possible to do acceptor photobleaching FRET.  Please contact me if  
you wish to use this microscope for FRET.  GFP FRAP should be done  
with the 488 and the FITC filter.

We will be updating the mfloor spectris to gfp/mCherry in the near  
future.  I will send a full list of filter sets when we come to do  
this to help you choose the best microscope for your work.  In the  
meantime, fixed work (esp. DAPI, FITC, TRITC, CY5) can still be done  
on the LMF spectris, and if these filters are required for LIVE CELL  
experiments, the mfloor spectris is configured with DAPI, FITC,  
TRITC, CY5, CFP, YFP, GFP.  This microscope also has 3 laser lines.

Cheers,
Sam