[FLIMfit-users] Issue with time-base

[Sean Warren]

Hi Tom,

Hope all's well

Yes there seems to be a problem with .msr files at the moment - I think there has been a change in the format.
We are working on fixing this with the bioformats guys.

You can use the "Load Time Point Calibration File" option to relabel the time axis. It expects a csv file with two columns with headers (header names are not important).
The first column should be the time points to be relabelled (i.e. the incorrect time points) and the second column should be matching correct time point.

Hope that helps in the short term - we should have the msr loading fixed soon

Cheers
Sean


________________________________

Dr. Sean Warren | Research Officer
Invasion and Metastasis
Cancer Division
Garvan Institute of Medical Research
The Kinghorn Cancer Centre, 370 Victoria Street, Darlinghurst, NSW 2010

T: + 61 (0)2 9355 5852 I E: s.warren at garvan.org.au

http://www.garvan.org.au/research/cancer/invasion-and-metastasis

________________________________
From: FLIMfit-users [flimfit-users-bounces at lists.openmicroscopy.org.uk] on behalf of Tom Robinson [Tom.Robinson at mpikg.mpg.de]
Sent: Wednesday, 23 March 2016 03:07
To: flimfit-users at lists.openmicroscopy.org.uk
Subject: [FLIMfit-users] Issue with time-base

Dear Sean (or other users),

I am having issues opening .msr files (generated using Imspector software on Abberior Instruments microscope with a B&H card) in FLIMfit. It can open them, then it asks which data set to select, and then it hangs on some process. I can, however, open the .msr files in Fiji (bio formats) and save them as ome-tiffs. FLIMfit is happy to open this format but all the time-base data gets lost.

Therefore 1) Is there a way to change the time-based within FLIMfit? Maybe using “load time point calibration file” (I couldn’t find info on this in the documentation. Or 2) be able to open the .msr files directly?

Thanks in advance,

Tom
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