[FLIMfit-users] FLIMfit help

[Munro, Ian]

Hi Jorge

Please note I m cc’ing this reply  to the FLIMfit-users list as I think this information could be  generally useful.

The “ success” field is only relevant for pixel-by-pixel fitting where it shows the percentage of pixels where the fitting process completed successfully.
In Global fitting there is only one fitting process (at least per segmented region) which either succeeds or not and the “success”  field is therefore not filled.

Further information about FLIMfit is available from https://github.com/openmicroscopy/Imperial-FLIMfit/wiki/GUI-walkthrough
or from either the paper or documentation which are linked in the first paragraph of
http://www.openmicroscopy.org/site/support/partner/flimfit.

Best Regards

Ian



On 29 Jan 2015, at 18:53, Jorge Palma Chavez <jorgepalma89 at tamu.edu<mailto:jorgepalma89 at tamu.edu>> wrote:

You are right Ian. I have been reviewing your paper "Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets" and I am wondering if you have an user guide where you specify all the parameters that you get from the FLIM fitting, for example Chi2 and W. Also I would like to know if I should get a success % greater than zero in the global fitting, as I get around 99% in the pixel-wise analysis.

Thanks again,

Jorge


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