[ome-users] Huygens OME exports are flipped

Thomas Pengo tpengo at umn.edu
Thu Jan 7 21:11:02 GMT 2016


Hi,

yes, I have seen that happen with Huygens with other image formats too
(ICS). I never contacted SVI about that but they may provide an
explanation. As it was one step of the workflow, I simply flipped it back
after opening it, but it's a workaround rather than a fix...

SVI might shed some additional light on the matter too!

Best
Thomas



________________________________________

Dr. Thomas Pengo
BioImage Analysis Specialist, Research Associate
University of Minnesota Informatics Institute
University of Minnesota
Cancer and Cardiovascular Research Building
2231 6th St SE
Minneapolis, MN 55455



On Thu, Jan 7, 2016 at 4:17 AM, Roger Leigh <rleigh at dundee.ac.uk> wrote:

> On 07/01/2016 01:15, Glen MacDonald wrote:
>
>> OME XML stacks created with Huygens Professional are flipped vertically
>> when opened with Bio-formats.
>> They are not flipped when re-opened with HuPro.
>>
>
> Dear Glen,
>
> Would it be possible to upload an example exported image demonstrating
> the problem using http://qa.openmicroscopy.org.uk/qa/upload/ so that we
> can take a look at the metadata and pixel data?
> The original image would also be useful as a reference though we may not
> be able to read it; a screenshot showing how the original file appears
> would be useful as well.
>
>
> Thanks,
> Roger
>
> --
> Dr Roger Leigh -- Open Microscopy Environment
> Wellcome Trust Centre for Gene Regulation and Expression,
> School of Life Sciences, University of Dundee, Dow Street,
> Dundee DD1 5EH Scotland UK   Tel: (01382) 386364
>
> The University of Dundee is a registered Scottish Charity, No: SC015096
>
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