[ome-users] possible bioformats bug--zeiss tiff files

[李长林]

Hi,


  I am new to the bio-formats. I have inherited a workflow that calls bioformats through FIJI. I find one possible bug using Bio-formats for reading Zeiss AxioVision TIFF and Zeiss AxioVision ZVI microscopy images.


  1. *.tif or *.tiff ? 


   The extensions in the plugin is different form the website （http://www.openmicroscopy.org/site/support/bio-formats5.3/supported-formats.html）（Fig.1 & Fig.2）
   


 2. I obtain microscope images with extensions of *.zvi or *.tif using Zeiss AxioVision, no  *.tiff. Therr is an error when reading series of Zeiss AxioVision TIFF (*.tif) images using Bio-formats（Fig.4）. If I change the  extensions of images to *.tiff manually, it will work, but sometimes error, too（Fig. 5）
 
      



 3. Reading *.zvi will change my data(Fig.6)
  
  


  Can you please provide some assistance - this is part of a high content assay so I really need to be able to run this in batch mode.


  Thank you for your help !


  Changlin Li


  Xiamen University,China
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