[ome-users] MetaMorph tif scaling

[Dotan Kamber]

Dears,

I'm facing an issue with scaling of MetaMorph tif images acquired with VisiView when opening with FIJI.
When separate tif images are saved as part of an "experiment " with accompanying .nd file the images are opened  with the correct scaling (nm/pixel) using the Bio-Formats importer when pointing to the tif images but not upon pointing to the .nd file or upon drag-and-drop to FIJI.
The scaling is also wrong when a single image (without accompanying .nd file) is opened both via drag-and-drop and the Bio-Formats Importer.
the same images are opened by Drag and Drop.
The following files have been uploaded –
Experiment_2_GFP.nd
Experiment_2_GFP_t1.tif
Experiment_2_GFP_t2.tif
Experiment_3_GFP.tif
For all the correct scaling is 0.645 microns per pixel
This value shows up in the parameters XCalibration and YCalibartion.
It seem that the wrong scaling is retrieved from the parameters XResolution and Yresoltion

Happy new year!
Dotan

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